Elucidating a fresh perspective on the interplay between exosomes and rheumatoid arthritis

www.frontiersin.orgJianan Zhao1,2,3,4†, www.frontiersin.orgBinbin Zhang5,6†, www.frontiersin.orgWanting Meng7 and www.frontiersin.orgJing Hu1*
1Department of Nephropathy, The Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
2Department of Rheumatology, Shanghai Guanghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China
3Guanghua Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai, China
4Arthritis Institute of Integrated Traditional and Western Medicine, Shanghai Chinese Medicine Research Institute, Shanghai, China
5Zhejiang University of Traditional Chinese Medicine, Hangzhou, China
6Department of Translational Medicine Platform, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
7Academy of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by chronic synovitis and the destruction of bones and joints. Exosomes are nanoscale lipid membrane vesicles originating from multivesicular bodies and are used as a vital means of intercellular communication. Both exosomes and the microbial community are essential in RA pathogenesis. Multiple types of exosomes from different origins have been demonstrated to have effects on various immune cells through distinct mechanisms in RA, which depend on the specific cargo carried by the exosomes. Tens of thousands of microorganisms exist in the human intestinal system. Microorganisms exert various physiological and pathological effects on the host directly or through their metabolites. Gut microbe-derived exosomes are being studied in the field of liver disease; however, information on their role in the context of RA is still limited. Gut microbe-derived exosomes may enhance autoimmunity by altering intestinal permeability and transporting cargo to the extraintestinal system. Therefore, we performed a comprehensive literature review on the latest progress on exosomes in RA and provided an outlook on the potential role of microbe-derived exosomes as emerging players in clinical and translational research on RA. This review aimed to provide a theoretical basis for developing new clinical targets for RA therapy.

Introduction
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by chronic persistent synovitis, systemic inflammation, and the presence of autoantibodies such as rheumatoid factor and anti-cyclic citrullinated peptide (anti-CCP) (Lee and Weinblatt, 2001; Scott et al., 2010). Untreated RA may lead to progressive disability and reduced quality of life, profoundly affecting the patient’s and family’s physical and mental health and increasing the burden on the whole family and society (Meier et al., 2013). For the clinical management of patients with RA, the first treatment principle is early detection, early intervention, and clinical remission with immunosuppression under the guidance of a physician (Meier et al., 2013). Currently, the main therapeutic drugs for RA include disease-modifying anti-rheumatic drugs, non-steroidal anti-inflammatory drugs, hormonal drugs, and new biologics to improve the treatment of the disease. Although certain clinical efficacy has been achieved, due to the complex factors of the disease itself, some patients still show no or poor/little response; therefore, the discovery of new disease targets and the development of clinical drugs with this is a top priority (Zhao et al., 2021).

RA risk factors include genetic, environmental, metabolic, immune, and microbial interactions, which lead to abnormal pathological features (Scott et al., 2010). Among these, multiple mechanisms of microbial community disorders are considered important pathological mechanisms (de Oliveira et al., 2017). Humans are multicellular organisms that co-exist as macroscopic beings with a diverse symbiotic microbial community, which is conservatively estimated to substantially exceed the number of constituent cells of humans (approximately 100 trillion). The microbial community also expresses more unique genes in its genome (Belkaid and Harrison, 2017). The existence of a large number of microbial communities contributes to the physiological functions of the body through a variety of mechanisms. The interaction between the microbial community and the host immune system is crucial to the local and systemic immune responses. Maintaining the balance of the microbiota is an essential factor for maintaining homeostasis (Lu et al., 2022). The primary association between gut microbes and RA is that preclinical and clinical patients with RA have abnormal changes in gut microbiota and gut microbial metabolites. These abnormal changes in microbial communities and metabolites may lead to abnormal immune recognition responses mediating the autoimmune response in RA.

The International Society for Extracellular Vesicles proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines suggest the use of “EVs” to generally denote a heterogeneous extracellular vesicle population, and “exosomes” are defined as small extracellular vesicles (Cocucci and Meldolesi, 2015; Théry et al., 2018). Multiple sources of extracellular vesicles exist in RA, which exerts effects on various immune cells. As for the extracellular vesicles themselves, they are an emerging and widely researched topic; they are being used as a diagnostic and therapeutic tool in the treatment of various diseases as a critical mechanism of intercellular communication, organ homeostasis, and disease (Kalluri and LeBleu, 2020). Microbe-derived exosomes are emerging stars in the field of exosomes that may play a crucial role in liver diseases. Their effects may include anti-inflammatory, intestinal barrier restorative, and immune modulatory effects, among other mechanisms (Zhang et al., 2022). Given the link between microbes and exosomes and RA, we present a summary and review of both and further examine the role of microbe-derived exosomes in the clinical management of RA to provide new theoretical support and options for the clinical management of RA.

Overview of the progress of exosomes and RA
Exosomes are defined as a set of vesicle-like structures released by all cell types, whose contain and exposure at their membrane surface protein and lipid components as well as nucleic acids originating from their original cell (Turpin et al., 2016). Exosomes are unable to replicate independently and are known by various names, such as microvesicles and microparticles (Miao et al., 2022). The extracellular vesicle can primarily be classified into two major categories, namely, ectosomes and exosomes. Ectosomes represent vesicles that result from the direct outward protrusion of the plasma membrane, generating entities such as microvesicles, microparticles, and large vesicles with sizes ranging from approximately 50 nm to 1 μm in diameter. Conversely, exosomes originate from endosomes and exhibit sizes within the range of approximately 40 nm–160 nm in diameter, with an average size of around 100 nm (Kalluri and LeBleu, 2020). The production of exosomes involves a double invagination of the plasma membrane and the creation of intracellular multivesicular bodies (MVBs) containing intraluminal vesicles (ILVs). The ILVs are eventually released as exosomes, which have a diameter ranging from approximately 40 nm–160 nm, through the process of MVB fusion with the plasma membrane and subsequent exocytosis (Kalluri and LeBleu, 2020). In accordance with the guidelines of The International Society for Extracellular Vesicles 2018 and consistency throughout the text, we use exosomes consistently throughout the text (Cocucci and Meldolesi, 2015; Théry et al., 2018). Exosomes mainly mediate intercellular messaging and are involved in numerous physiological processes, such as inflammation, immune response, angiogenesis, cellular stress, cellular senescence, cell proliferation, and differentiation (Turpin et al., 2016). Transcriptomic analysis revealed that exosomes transport a variety of cargoes, including many functional small coding miRNAs, messenger RNA (mRNA), and non-coding RNA (ncRNAs) (Lee et al., 2007; Kaparakis-Liaskos and Ferrero, 2015). Proteomic analysis has demonstrated that exosomes also transport other cargo, including membrane-bound proteins, enzymes (e.g., autolysins), toxins, polysaccharides, and peptidoglycans (Lee et al., 2007; Kaparakis-Liaskos and Ferrero, 2015). There exist different patterns of extracellular vesicle in RA. For instance, Hirotaka et al. found significant differences in the serum exosomes protein profile between active RA patients, osteoarthritis (OA) patients, and healthy individuals. Specifically, RA patients exhibited higher levels of TLR3 and pro-neuregulin-3 proteins, which may reflect the hyperactive state of RA fibroblast-like synoviocytes (FLS) (Tsuno et al., 2018). Proteomic analysis of plasma exosomes from RA patients and healthy controls identified 32 upregulated proteins and 5 downregulated proteins. Bioinformatics analysis indicated that these differentially expressed proteins interact with each other and mainly participate in inflammation pathways affecting RA (Qin et al., 2021). Results from a systematic review and meta-analysis showed that the total number of exosomes in both plasma and synovial fluid was increased in RA patients compared to healthy individuals, which is mainly associated with the release of cytokines and chemokines(Schioppo et al., 2021).Compared with osteoarthritis, gout, and axial spondyloarthritis, it was found that 28 proteins were uniquely upregulated in exosomes derived from RA synovial fluid. Bioinformatics analysis indicated their significant involvement in “serine-type endopeptidase inhibitor activity” and “complement and coagulation cascades” (Huang et al., 2022a). Therefore, We demonstrate the therapeutic potential of exosomes from different sources by elucidating their effects on different immune cell populations in RA.

Potential effects of different sources of exosomes on fibroblast-like synovial cells in RA
RA fibroblast-like synovial cells (FLS) are a crucial population responsible for abnormal synovial proliferation, inflammation, and bone destruction. Exosomes originating from RA FLS themselves are shown to promote their abnormal proliferation and synovial hyperplasia by carrying certain substances secreted by the cells. RA FLS is an essential group of cells in RA that causes abnormal synovial proliferation, inflammation, and bone destruction. Exosomes from RA FLS promote their abnormal proliferation and synovial proliferation by carrying some substances secreted by themselves. For example, Exosomes from RA FLS contain membrane-bound forms of tumor necrosis factor-α (TNF-α), which promotes the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-KB) and induction of membrane-type matrix metalloproteinase (MMP)-1 in RA FLS (Zhang et al., 2006). RA FLS overexpress and releases cluster of differentiation (CD) 13, which acts mainly as an intracellular soluble molecule and is encapsulated in exosomes, enhancing RA FLS proliferation and migration by acting on itself. Inhibition of CD13 can inhibit this effect (Morgan et al., 2016). Transforming growth factor beta (TGF-β) stimulates FLS to produce an inhibitor of DNA binding 1, which is passed to endothelial progenitor cells and human dermal microvascular endothelial cells and activates the c-Jun N-terminal kinases (JNKs) signaling pathway to promote angiogenesis through exosomes from RA FLS (Edhayan et al., 2016). Some exosomes from other sources have different effects on promoting and inhibiting RA FLS proliferation and inflammation, which depend on the functional roles of the cargo types carried by the exosomes. For example, the peripheral blood mononuclear cell-derived exosome lncRNA nuclear-enriched abundant transcript 1 (NEAT1) targets and regulates the miR-23a/mouse double minute 2 homolog (MDM2)/sirtuin 6 (SIRT6) axis to promote proliferation and inflammation in RA FLS (Rao et al., 2020). However, T cells release large amounts of derived exosomes, miR-204-5p, to transfer to FLS and inhibit cell proliferation (Wu et al., 2022). It is noteworthy that exosomes derived from mesenchymal stem cells have been extensively studied and proven to be beneficial for RA, including: i) Reducing vascular endothelial growth factor (VEGF) expression and angiogenesis: For example, Synovial mesenchymal cell-derived exosomes inhibit miR-485-3p/signal transducer and activator of transcription 3 (STAT3) by transmitting circRNA-circEDIL3 to reduce VEGF expression and angiogenic activity in RA FLS induced in vitro and reduce the extent of arthritis in collagen-induced arthritis (CIA) models (Zhang et al., 2021). Similarly, mesenchymal stem cell-derived miR-150-5p targeting membrane-type matrix metalloproteinase 14(MMP14), and VEGF reduced angiogenic activity and clinical arthritis scores in arthritic mice (Chen et al., 2018). ii) Inhibiting synovial inflammation, synovial hyperplasia, and bone destruction. For example, human umbilical cord mesenchymal stem cells (MSCs)-derived exosome miR-140-3p inhibited inflammation, oxidative stress, and fibrotic response and suppressed synovial proliferation by silencing serum- and glucocorticoid-induced kinase 1 (SGK1) in CIA models (Huang et al., 2022b). MSCs-derived exosomes of circFBXW7 and miR-320a can inhibit cell proliferation, migration, inflammatory response, and bone damage in RA FLS by directly targeting miR-216a-3p to upregulate histone deacetylase 4 (HDAC4) and C-X-C motif chemokine 9 (CXCL9) (Meng and Qiu, 2020; Chang and Kan, 2021). Chondrogenic bone marrow mesenchymal stem cell-derived exosomes directly inhibit RA FLS pro-inflammatory factors, MMP production, and activation of mitogen-activated protein kinase (MAPK) and NF-KB pathways and ameliorate arthritis in CIA mice (Ma et al., 2022).

Potential effects of different sources of exosomes on T helper 17 cells, regulatory T cells, and regulatory B cells in RA
T helper 17 (Th17) cells, a subset of T cells, are a pro-inflammatory cell population in RA. Exosomes from different sources can affect disease progression by influencing the proliferation of CD4+ T cells and their differentiation towards Th17 cells. For example, autoimmune T cells engulfing RA FLS-derived exosomes cause increased resistance to apoptosis and prolong the survival time (Zhang et al., 2006). The lncRNA NEAT in RA blood exosomes promotes CD4+ T cell proliferation and Th17 cell differentiation by regulating the miR-144-3p/rho associated coiled-coil containing protein kinase 2(ROCK2)/WNT axis for disease progression (Liu et al., 2021). Interleukin (IL)-17, a pro-inflammatory cytokine secreted by Th17 cells, is an important pro-inflammatory factor in RA. Exosomes derived from human gingival mesenchymal stem cells inhibited IL-17A secretion and promoted the secretion of the anti-inflammatory factor IL-10, reduced bone erosion, and improved arthritis in CIA models and in vitro CD4+ T cell co-culture models (Tian et al., 2022). This further supports the link between the mouth and joints. A reduction in T cell differentiation towards the regulatory T (Treg) cells is a key factor in inflammation in RA, as Treg cells are an important cell population that suppresses Th17 differentiation. The effects of exosomes from different sources on Treg cell may be bidirectional. On the one hand, they may promote inflammation by inhibiting Treg cells. For example, exosomes derived from RA FLS lncRNA HOXA distal transcript antisense RNA (HOTTIP) negatively regulate miR-1908-5p and positively regulate STAT3, leading to upregulation of forkhead box P3 (FOXP3) and retinoid-related orphan receptor gamma (RORγt) expression and Th17/Treg cell ratio imbalance (Yao et al., 2021). Similarly, the RA joint cavity exhibited an overall hypoxic microenvironment owing to the over-proliferation of FLS. miR-424 in exosomes derived from RA FLS significantly induced Th17 differentiation and inhibited Treg cells differentiation under hypoxic conditions (Ding et al., 2020). On the other hand, they may promote the suppression of inflammation by enhancing Treg cells. For example, exosomes from MSCs can significantly suppress T- and B cell immune responses, increase Treg cells in vitro, and significantly suppress inflammatory responses in CIA models (Cosenza et al., 2018). miR-146a/miR-155 in MSCs exosomes suppress aberrant immune responses by increasing FOXP3, RORγt, TGF-β, IL-10, IL-17, and IL-6 expression and increasing the Treg cell population in CIA models (Tavasolian et al., 2020).

In addition, autoimmune B cells are the source of many autoantibodies, and regulatory B (Breg) cells suppress inflammation by secreting IL-10 and other anti-inflammatory factors in RA. Exosomal prostaglandin E2 secreted by granulocytic myeloid-derived suppressor cells can significantly upregulate glycogen synthase kinase-3 beta (GSK-3 beta), and adenosine triphosphate response element-binding protein phosphorylation can promote IL-10+ Breg production to suppress inflammation in CIA models (Wu et al., 2020).

Potential effects of different sources of exosomes on osteoblasts, chondrocytes, and osteoclasts in RA
The destruction of osteoblasts and the excessive production of osteoclasts are important factors in bone destruction in RA. On the one hand, some exosomes may promote the process of bone destruction. Reacher found that the levels of exosomal receptor activator of nuclear factor kappa-B ligand (RANKL) isolated from the synovial fluid of patients with RA were significantly higher than those of patients with several other types of arthritis and induced higher numbers of osteoclasts involved in bone destruction (Song et al., 2021). Smoking is considered a risk factor for RA (Zhao et al., 2021). Cigarette smoke promotes exosomal miR-132 secretion by Th17 cells, downregulating cyclooxygenase-2 (COX2) to promote osteoclastogenesis, and knockdown of miR132 in an arthritis model reduced the number of osteoclasts (Donate et al., 2021). Exosomes originating from RA FLS affect the RANKL/RANK/osteoprotegerin (OPG) pathway by transporting miR-106b to chondrocytes and inhibiting pyruvate dehydrogenase kinase isozyme 4 (PDK4), which inhibits chondrocyte proliferation and migration and accelerates apoptosis. In vivo inhibition of miR-106b improves arthritic symptoms in a CIA model (Liu et al., 2020). On the other hand, the researcher also found that in vitro osteoblasts can phagocytose miR-486-5p of exosomes originating from RA FLS and increase miRNA expression, which inhibits transducer of erb-b2 receptor tyrosine kinase 2, 1(TOB1) and activates the bone morphogenetic protein (BMP)/suppressor of mothers against decapentaplegic (SMAD) pathway to promote osteoblast differentiation, which is potentially beneficial in RA treatment (Chen et al., 2020).

Potential effects of exosomes from different sources on macrophages in RA
Research on exosomes from different sources for their effects on macrophages in RA is still relatively limited. Secretion of miR-let-7b-containing exosomes by macrophages themselves promotes the differentiation of M1-type pro-inflammatory macrophages in RA joint inflammation by binding Toll-like receptor 7 through the GU structural domain (Kim et al., 2016). Additionally, serum-derived exosome miR-6089 and miR-548a-3p can regulate macrophage proliferation and differentiation, as well as inflammatory factor production, by targeting Toll-like receptor 4 (TLR4) (Wang et al., 2018; Xu et al., 2019). This is also in line with the pro-inflammatory role of macrophages themselves. miR-223 from exosomes sourced from bone marrow mesenchymal stem cells inhibits the release of IL-1β, TNF-α, IL-18 and the activation of the NOD-like receptor protein 3 (NLRP3) to attenuate macrophage inflammation (Huang et al., 2022c). Notably, a new type of nanovesicle was made by combining the membrane of an M1 macrophage with nanovesicles that mimic exosomes from M2 macrophages (Zhao et al., 2022). The nanovesicle has the ability to effectively reduce inflammation by binding to proinflammatory molecules and releasing anti-inflammatory mediators (Zhao et al., 2022). This comprehensive anti-inflammatory activity makes it a powerful tool for treating inflammatory diseases such as rheumatoid arthritis (Zhao et al., 2022) Figure 1.

Figure 1
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FIGURE 1. Association of multiple exosomes with RA. The extracellular vesicle can primarily be classified into two major categories, namely, ectosomes and exosomes. Ectosomes represent vesicles that result from the direct outward protrusion of the plasma membrane, generating entities such as microvesicles, microparticles, and large vesicles with sizes. The production of exosomes involves a double invagination of the plasma membrane and the creation of intracellular multivesicular bodies (MVBs) containing intraluminal vesicles (ILVs). The ILVs are eventually released as exosomes through the process of MVB fusion with the plasma membrane and subsequent exocytosis. Blood, FLS, mesenchymal stem cells, and other cell-derived exosomes are all associated with RA through different mechanisms. Gut microbe-derived exosomes are mainly involved in altering the permeability of the intestinal barrier and transporting cargo to the extraintestinal system affecting the immune system, and may be a novel target for future RA therapy.

The association of gut microbes with RA
The intestine is the organ with the largest surface area in the body. Various microorganisms are colonized in the intestine, and these microbial communities and their products play a vital role in the immune system (Round and Mazmanian, 2009). The pathophysiological relevance of gut microbes in RA may vary (Table 1). The possible mechanism by which gut microbes influence autoimmunity is as follows:

(i). Intestinal barrier dysfunction: Intestinal barrier dysfunction caused by intestinal microbial disorders is an important checkpoint for inflammation in CIA models and RA patients, and the use of the intestinal barrier modulator butyrate can effectively inhibit arthritis (Tajik et al., 2020). There are abnormal changes at the phylum or genus level in various microorganisms in RA. For example, the α- and β-diversity indices of the microbiome are significantly reduced in mice with experimental RA, and they have an elevated proportion of Firmicutes and Bacteroidetes. Four core genera, including Eubacterium_Ventriosum, Alloprevotella, Rikenella, and Treponema, were reduced in fecal and mucus content (Nguyen et al., 2022).

(ii). Molecular mimicry, in which gut microbes or metabolites trigger an autoimmune response of immune cells. The epitopes of Prevotella sp., Parabacteroides sp., and Butyricimonas sp. have obvious homology sequence with two autoantigens, N-acetylglucosamine-6-sulfatase, and filamin A, presented by major histocompatibility complex II cell surface receptor in RA (Pianta et al., 2017).Pattern recognition receptors on the surface of antigen-presenting cells constantly recognize and respond to potential antigens in the intestinal lumen, which often include some autoantigens. For example, antigen-presenting cells recognize gut microbes and metabolites (such as LPS and polysaccharide analogs) as potential antigens, leading to downstream T cell cascade differentiation and immune responses, which may initially result in local immunity. However, they can cause systemic autoimmune responses with lymph nodes or humoral circulation (Horta-Baas et al., 2017).

(iii). Regulation of multiple immune cells by multiple metabolites derived from intestinal microorganisms: mainly short-chain fatty acids (butyrate, acetate) and amino acid metabolites (tryptophan metabolism), which can also affect systemic immunity through abnormal intestinal barrier function into the circulation, and the detailed mechanisms can be found in the excellent review (Yang and Cong, 2021). In addition to changes in the microbial community, there were changes in its primary metabolites (short-chain fatty acids, bile acids, amino acids, and tryptophan metabolites) in RA. For example, the Firmicutes/Bacteroidetes ratio was low at the gate level in experimental arthritic mice with adjuvant induced arthritis (AIA). At the genus level, there was an abundance of Akkermansia, Prevotella, Bacteroides, Enterococcus, Dorea, and Jeotgalicoccus and a lower abundance of Oscillospira. Metabolites included acetic acid, propionic acid, butyric acid, valeric acid, capric acid, lactic acid, primary bile acids, secondary bile acids, conjugated bile acids, lipopolysaccharides (LPS), and conjugated bile acids (Cheng et al., 2022).

Table 1
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TABLE 1. Abnormally altered microbial communities in RA.

Abnormal responses to RA can be ameliorated by interventions to improve abnormal microbial changes including some RA clinical agents. For example, increased beneficial microbiota in patients with RA treated with the anti-tumor necrosis factor antibody etanercept (Picchianti-Diamanti et al., 2018). Methotrexate (MTX) may predominantly affect Bacteroidetes, and transplantation of microorganisms from patients with RA receiving MTX into germ-free mice under inflammatory stimuli results in a reduced inflammatory response, including reduced activation of multiple immune cells (Nayak et al., 2021). Therefore, correcting the abnormal changes in gut microbiota and metabolites in RA is one of the important research directions of the gut-joint axis. It should be noted that exosomes derived from gut microbiota may be an important component of the gut-joint axis, but as relevant research is still relatively scarce, we will speculate and prospect on the relevant theoretical basis in the following, in anticipation of providing a theoretical basis for future experimental research.

Future perspectives: gut microbe-derived exosomes may serve as a critical link in the gut-joint axis
Gut microbe-derived exosomes have been suggested as vital mediators of immune transmission in the intestinal mucosa (Kaparakis-Liaskos and Ferrero, 2015; Olsen and Amano, 2015). There are four main types of exosomes derived from intestinal microorganisms: outer membrane vesicles, outer inner membrane vesicles, explosive outer membrane vesicles produced by gram-negative bacteria, and cytoplasmic membrane vesicles produced by gram-positive bacteria (Zhang et al., 2022). In regards to how exosomes from gut microbiota participate in the gut-joint axis, we have the following speculations:

(i). The intact intestinal barrier can prevent excessive activation of the mucosal immune system and inflammatory responses in the intestine (1). For example, exosomes secreted by the probiotic E. coli strain Nissle 1917 or commensal E. coli strain ECOR12 can promote the secretion of various inflammatory cytokines and chemokines, including IL-6, IL-8, TNF-α, and chemokine (C-C motif) ligand 3 (CCL3), from peripheral blood mononuclear cells in vitro and were unable to elevate these factors in an in vitro model of intestinal mucosa co-cultured with Caco-2/peripheral blood mononuclear cells, suggesting that this intact intestinal barrier prevents transitory activation of intestinal mucosal immunity and inflammatory responses (Fábrega et al., 2016).

The gut microbiota and its metabolic products can exert their effects beyond the intestinal area through various complex mechanisms. For example, studies have reported that probiotic intake can improve abnormal disease behaviors through communication from the peripheral immune system to the brain, such as immobility, which is commonly seen in patients with RA, by mechanisms that may involve reducing the number of TNF-α signals in the peripheral immune system that are recruited to monocytes in the brain in response to inflammation and the subsequent activation of brain microglia (D’Mello et al., 2015). Complicated lung disease is a leading cause of death in patients with RA. segmented filamentous bacteria (SFB) induce pulmonary autoimmunity by amplifying Th17 cells expressing dual T cell receptors, recognizing their antigenic epitopes, and inducing pulmonary injury in combination with pulmonary expression of C-C motif chemokine ligand 17 (CCL17) (Bradley et al., 2017).

Disturbances in the microbial communities or metabolic can lead to local inflammatory responses in the intestine and increase intestinal permeability (Alghamdi et al., 2021), which may contribute to the systemic diffusion of autoantibodies and the inflammatory response of the joints in RA. For example, the overall gut microbial diversity was reduced in patients with RA and correlated with disease duration and autoantibody levels, and Collinsella was significantly associated with the production of pro-inflammatory cytokines and altered intestinal permeability (Chen et al., 2016). In addition, the gut microbial metabolite short-chain fatty acid butyrate was reduced in patients with RA and animal models of arthritis, which may also lead to altered intestinal permeability (Rosser et al., 2020).

(ii). Autoantibody production is a distinctive characteristic of RA, and these autoantibodies may form immune complexes in the joints, leading to chemokine and cytokine secretion through complement activation or direct activation of immune cells, thereby attracting immune cells and enhancing the immune response, ultimately resulting in chronic inflammation and bone destruction (van Delft and Huizinga, 2020).The intestine may be an essential source of autoantibody production. Gut microbe-derived exosomes can be found in tissues and blood throughout the body and interact with multiple immune cells, suggesting that gut-derived exosomes can act as communicators between the intestinal and parenteral systems (Jones et al., 2020; Bittel et al., 2021). They may act as carriers to transport a variety of substances to extraintestinal systems, including joints. When multiple cells release intracellular mitochondrial DNA due to injury, dendritic cells can recognize this DNA to drive Th1 cell polarization in autoimmunity. Exosomes derived from intestinal microbes may initiate an immune response by transporting bacterial DNA, leading to the recognition of CpG motifs by dendritic cells (Lin and Zhang, 2017). In addition, there is a significant increase in gram-negative bacteria in RA, which induces the activation of Th17 cells and immune responses to autoantigens. This is also consistent with the hypothesis of an additional RA-gut link, in which toxic metabolites secreted by intestinal gram-negative bacteria enter the circulation, leading to systemic immunity and autoinflammation (Brusca et al., 2014; de Oliveira et al., 2017). Therefore, exosomes derived from intestinal microbes may also serve as important carriers for transporting self-antibodies produced in the gut to other parts of the body, leading to the inflammatory and bone-destructive processes. Research suggests that synovial-derived exosomes in RA can transport self-antibodies to enhance the autoreactive immune response through the process of antigen presentation (Alghamdi et al., 2021). The symptoms of arthritis in mice were greatly reduced when exposed to sterile conditions, in conjunction with a notable decrease in levels of serum autoantibody titers, splenic autoantibody-secreting cells, and Th17 cells. However, the loss of Th17 cells and autoantibody production in the lamina propria of the small intestine was restored when a single SFB was introduced (Wu et al., 2010).

In addition, miRNA-microbe interactions have been explored as a crucial mechanism in various diseases, and miRNA expression patterns in mouse and human fecal and intestinal lumen contents have also been explored (Liu et al., 2016a). Among them, gut microbe-derived exosomes can act as communication mediators to regulate host gene expression and even affect the microbial community (Liu et al., 2016a; Dong et al., 2019; Tavasolian and Inman, 2021). For example, transplantation of gut microbe-derived exosomal miRNAs from wild-type mice ameliorates intestinal barrier dysfunction and inflammation in systemic lupus erythematosus (Liu et al., 2016a). In the context of RA, miRNAs occupy an important position (Chang et al., 2022) and may also interact with the gut-joint axis through exosomes derived from gut microbes.

(iii). The link between gut microbes and joint inflammation has been established and in conjunction with the role of exosomes in RA, we speculate that gut microbe-derived exosomes are involved in the gut-joint axis:

As previously mentioned, there are pathological changes in gut microbiota in RA, including alterations in microbiota abundance and composition, which may result in the production of abnormal bioactive substances (such as metabolites), and impact the pathological features of RA in areas beyond the gut through various mechanisms (such as impaired intestinal barrier function). The abnormal changes in gut microbiota may transmit these aberrant bioactive substances through extracellular vesicles and lead to the subsequent progression of inflammation, angiogenesis, and bone destruction in RA, but further experiments are needed to investigate this phenomenon.

Discussion
Different sources of exosomes may affect various immune cells in RA through multiple mechanisms, primarily including FLS, T cells, macrophages, osteoclasts, osteoblasts, and chondrocytes. The effects of exosomes from different sources on immune cells depend on the types and functions of the cargoes they carry. It is noteworthy that exosomes derived from mesenchymal stem cells appear to have an overall beneficial effect on RA, which is a promising direction for future development. Abnormal changes in the intestinal flora and multiple sources of exosomes have been observed to have multiple effects on the pathophysiological processes of RA. Although the quantitative proportions of gut microbiota, the most extensive symbiotic system in the human body, are abnormally altered in RA with specific alterations at the phylum and genus levels, metabolites have also been intensively studied and have given birth to many hypotheses. There are still many issues that need to be further elucidated, for example, to clarify the clear linkage of one or several gut-joint axes, widely demonstrated by experiments; nevertheless, there is no doubt that the primary principle is to maintain a reasonable balance between the microbial community and the organism and to prevent ecological imbalances, which may prevent harmful consequences in the gut as well as in the extraintestinal system. Based on the present hypothesis, using samples of preclinical models and clinical patients combined with proteomics, metabolomics, metagenomics technologies, and bioinformatics are essential tools for analysis. Finally, adopting multiple basic molecular biologies and microbiology approaches for continued validation is a necessary direction for the future. Due to the current technical limitations of exosomes, there are still various limitations for their isolation, extraction, and classification, which are proposed to be solved in the future. This is a blank field in the field of RA for microbe-derived exosomes. Gut-derived exosomes may serve as a crucial bridge between gut microbiota and RA. Nevertheless, we have provided reasonable hypotheses on the link between microbe-derived exosomes and the disease based on the association of microbial communities and exosomes with RA. Unfortunately, current relevant studies do not provide sufficient depth; therefore, we can only make appropriate hypotheses on the mechanism of action based on existing studies, mainly related to the alteration of intestinal permeability and possible transport of cargo to extraintestinal systems. Further experimental corroboration is required for in-depth studies. The future direction of our team is to build on this knowledge and gradually enhance future strategic research on the gut-joint axis, which is of great economic and clinical value.

Author contributions
JZ and BZ is responsible for the collection, collation, and writing of the original manuscript. MW is responsible for the collection. JH are responsible for the concept development, revision, and manuscript review. All authors reviewed and accepted the final version.

Funding
This work was funded by the Famous Traditional Chinese Medicine” Talent Training Plan of the Seventh People’s Hospital affiliated to Shanghai University of Traditional Chinese Medicine (MZY2021-01); “Medical Craftsman” Talent Training Plan of the Seventh People’s Hospital affiliated to Shanghai University of Traditional Chinese Medicine (GJ2021-06); Pudong New Area Traditional Chinese Medicine Brand Multiplication Plan—Chronic Nephropathy (PDZY-2021-0302), Construction of He Liqun’s famous TCM studio. Project supported by Shanghai Municipal Science and Technology Major Project (ZD2021CY001).

Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note
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黄蛭益肾胶囊治疗慢性肾脏病专家共识

何立群
《黄蛭益肾胶囊治疗慢性肾脏病专家共识》编写组上海中医药大学附属曙光医院
摘要:<正>慢性肾脏病(chronic kidney disease,CKD)是指各种原因引起的慢性肾脏结构和功能障碍(肾脏损害病史大于3个月),包括肾小球滤过率(GFR)正常和不正常的病理损伤、血液或尿液成分异常,及影像学检查异常;或不明原因GFR下降(<60 ml/min·1. 73 m2)超过3个月。是目前发病率较高的一种危害人体健康的慢性疾病,如不能进行早期诊断及干预,CKD易进展至终末期肾病(ESRD),并引发多种严重并发症,严重危害人类健康。随着我国人口老龄化及高血压、糖尿病患病率增多,CKD的发病率呈逐年上升的趋势。如何有效防治CKD、减轻CKD危害,已成为广大医务工作者研究的重要课题[1],是全球关注的重要公共卫生问题。
专辑:医药卫生科技
专题:泌尿科学

何立群医案(三)

孙某,男,80岁

初诊时间:2022-09-16

主诉:肌酐升高8年余,乏力纳差1周

现病史:患者2014年因痛风就诊于我科,入院完善相关检查,查(2014-03-26)ECT:左侧肾小球滤过功能轻度受损,右侧肾小球滤过功能正常(左肾GFR31.9ml/min,右肾GFR37.94ml/min,总GFR69.83ml/min),(2014-4-2)血常规:WBC5.08×10^9/L,N53.1%,HB139g/L,CRP:5mg/l;肾功能:尿素氮5.86mmol/L,肌酐82.6umol/L,尿酸497.2umol/L,胱抑素C1.09mg/L;电解质:血钙2.6mmol/L,诊断慢性肾脏病2期。后未定期门诊随访。1周前患者无明显诱因下出现乏力纳差,休息后未见明显缓解,遂就诊于金高中西医结合医院,查肾功能(2022.09.09):肌酐182,UA 660,BUN 12.33。现患者为求进一步诊疗,由门诊拟“慢性肾脏病4期”收治入院。患者既往有高血压病40余年,最高血压180+/100+mmhg,目前口服可多华、替米沙坦氢氯噻嗪、美托洛尔、吲达帕胺控制血压,血压控制欠佳。有冠心病病史,口服单硝酸异山梨酯、阿托伐他汀治疗。有前列腺增生病史。2015年行右侧甲状腺切除术。

刻下:面色萎黄,肢体困重,胸闷气急,腰酸乏力,纳呆泛恶,夜寐欠安,小便短少,夜尿频数,大便干结。舌淡胖,舌下脉络迂曲,苔少,脉沉细。

西医诊断:1.慢性肾脏病4期2.冠状动脉粥样硬化性心脏病3.痛风4.高血压病3级(极高危)5.前列腺增生

中医诊断:肾衰病(脾肾亏虚,瘀血内阻)

治则:补益脾肾,活血化瘀

处方:

党参30        黄芪30        赤芍15          蜜麸炒白芍15

当归15        熟大黄9       炒王不留行30    炒车前子(包煎)30

炒芥子10      蜜麸炒白术15  防风12          猪苓15

白茯苓15      牛膝15        燀桃仁(捣碎)15   红花10

丹参20        蒲公英30      白花蛇舌草30    肉苁蓉15

菟丝子15      芡实15        覆盆子15        陈皮10

川佛手12      金蝉花12      蜜麸炒苍术15

14剂,每日1剂,水煎2次,取汁混合,分两次早晚温服。并嘱其饮食调理,低脂、低糖、低盐、优质蛋白质饮食,避风寒,慎起居,调饮食,畅情志。

方解:该方中以党参补中益气,黄芪补气升阳,赤芍清热凉血,白芍养血调经,当归补血活血,熟大黄峻下攻积,王不留行活血通经,车前子利尿通淋,芥子温肺化饮,白术补气健脾,防风祛风解表,猪苓利水渗湿,白茯苓益气健脾,金蝉花祛风益肾,牛膝补肝肾,强筋骨,桃仁活血祛瘀,红花祛瘀止痛,丹参凉血消痈,蒲公英、白花蛇舌草清热解毒,肉苁蓉补肾阳,菟丝子补肾固精,芡实益肾固精,覆盆子固精缩尿,陈皮理气健脾,佛手疏肝解郁,苍术燥湿健脾。

按语:慢性肾脏病发展至终末期,病因多涉及多个脏腑,病机比较复杂。病危在肾而又与脾胃密切相关。在病机的认识上多是以脏腑虚损(脾肾)而复感外邪,加之情志、劳累等因素,而致正气虚损,浊邪塞滞。针对于这样的患者,何立群教授长期临床经验总结肾病一号方、二号方拟做底方。一号方多适用于脾肾亏虚,正虚为主的患者,二号方适用于邪盛为主。邪实多见于内外风、水湿、浊毒、瘀血等。该病人以二号方为底,以其大便干结而浊毒内盛为主。加之患者瘀血内阻,辅之以活血化瘀、健脾理气。

何立群医案(二)

林某,女,55岁。

初诊时间:2022年8月30日。

主诉:腰酸伴泡沫尿3年余,加重1周

现病史:3年前患者因过度劳累后出现腰酸伴泡沫尿,遂至当地医院就诊,西医诊断慢性肾 炎,服用西药治疗后症状减轻,未予重视。一周前因劳累,出现腰痛、双眼睑、双下肢浮肿, 逐渐加重,来院查尿常规:尿蛋白(+++),24h微量蛋白2.273g,肾功能正常。

有糖尿 病病史5年余,目前胰岛素治疗;高血压病史30年,目前服用拜新同。空腹血糖 13.2mmol/L。

刻诊:患者腰痛腰酸,神疲乏力,肢体浮肿,乏力明显,小便短少,大便溏稀,舌胖大, 质淡,苔黄腻,舌下络脉青紫,脉沉细无力。

西医诊断:慢性肾炎。

中医诊断:水肿病(脾肾两虚,兼风湿瘀滞证)

治则:健脾化湿,温肾固精,祛风通络

处方:

党参30g    黄芪30g    山茱萸15g   山药15g

生地15g    枸杞子15g  茯苓15g     知母12g

黄柏12g    炒白术15g  防风12g     蝉蜕6g

蚕茧壳6g   薏苡根30g  僵蚕12g     炒牛膝15g

丹参30g    川断15g    杜仲15g     桃仁12g

芡实12g    覆盆子15g  荆芥12g

14剂。每日1剂,水煎2次,取汁混合,分2次早晚温服。

并嘱其饮食调理,低脂、低糖、低盐、优质蛋白质饮食,避免劳累和感冒,不要熬夜、按时起卧,适度节制性生活。

按语:本医案中患者感受风邪,内舍于肺,肺失宣降,水道不通,以致风水相搏,泛滥肌肤,故见双眼睑及下肢浮肿。患者中阳不振,健运失司,运化失常,以致下焦水邪泛滥,脾升清运化功能失调,则体内水湿代谢异常,日久湿邪内停,且湿性重浊、极易困脾、故见腰以下尤甚,脾虚生湿,运化无力,故见大便溏薄,舌苔厚腻:脾虚气血生化乏源,阳不温煦,故见神疲乏力;水湿之邪,浸渍肌肤,壅滞不行,以致肢体浮肿不退,水湿日增而无出路,横溢肌肤,所以肿势日重。脾肾两虚,水液运行失其常道,故见双足浮肿,日久血瘀,故见腰痛腰酸,舌下络脉青紫;脉沉细无力,乃是脾肾两脏亏虚,湿热瘀滞之象。

何立群医案(一)

李某,女,65岁
初诊:2022年8月22日。
主诉:发现血尿4年余。

现病史:2018年5月8日因感寒至当地医院就诊,查尿常规提示尿隐血:(+++),尿蛋白(+++),2013年8月22日24h蛋白尿定量0.734g。肾功能:尿素氮:6.5mmol/L,肌酐74μmol/L,血尿酸304μmol/L。双肾动脉彩超提示双肾动脉未见狭窄,血流通畅。患者一直在当地医院就诊,期间服用中药及中成药(具体用药剂量不详),症情有所缓解。2022年6月7日就诊于上海交通大学附属瑞金医院,查尿常规:尿潜血(+++),尿红细胞(+++),尿蛋白(++)。24h蛋白尿定量1.765g,尿素氮:8.5mmol/L,肌酐104μmol/L,血尿酸504μmol/L。肾活检组织学报告:IgA肾病(局灶节段硬化),Oxford评分:M0、E0、S1、T0。诊断为IgA肾病,予对症治疗后,病情未明显缓解。2022年8月22日前来就诊。

初诊症见:精神疲倦,腰部酸软,纳可,眠欠佳,双下肢轻度凹陷性水肿,夜尿2次,手足心出汗,肉眼血尿,尿中泡沫,舌淡红,有裂纹,苔微腻,脉沉滑。

西医诊断:IgA肾病(局灶节段硬化),

中医诊断:尿血(脾肾两虚,湿浊内阻)。

治法:补益脾肾,利湿化浊。

方药:童氏健脾益肾方加减。

处方:

党参30g&nbsp;&nbsp;&nbsp;&nbsp; 黄芪各30g&nbsp;&nbsp; 白术15g&nbsp;&nbsp;&nbsp;&nbsp; 防风15g
小蓟30g&nbsp;&nbsp;&nbsp;&nbsp; 荠菜花15g&nbsp;&nbsp; 白茅根30g&nbsp;&nbsp; 山茱萸15g
枸杞子15g&nbsp;&nbsp; 地黄15g&nbsp;&nbsp;&nbsp;&nbsp; 金樱子30g&nbsp;&nbsp; 藿香10g
佩兰10g7剂,每日1剂,水煎早晚温服。

2022年8月30日二诊:精神疲倦,腰部酸软,手足心出汗等症状较前明显改善,尿中泡沫仍多,双下肢轻度凹陷性浮肿,舌淡红,有裂纹,苔微黄腻,脉沉滑。尿常规:尿红细胞(++),尿潜血(+),尿蛋白(++),24h蛋白尿定量1.325g。肾功能:尿素氮:7.3mmol/L,肌酐94μmol/L,血尿酸404μmol/L。

辨证:脾肾两虚,湿热内阻。

上方去藿香、佩兰,

加半夏9g、蝉蜕6g、蚕茧壳9g、薏苡根30g。

按:患者年过六旬,卫气不固,外邪入侵,病邪由表及里,损伤脾肾,肾脏封藏失职,不能固摄,脾气升清功能失调,营养精微物质不能布散全身,清阳下陷,精微外流,予以童氏健脾益肾方加减补益脾肾,利湿化浊,以达固摄精微,培补先后天。服药7剂,尿浊仍在,续用化浊固摄之药;潜血得减但犹存,续用敛血之品;但患者体质湿热,滋腻太过,加之补阳之药化热,宜稍减而不碍本意;其余症状均比之前改善,配伍得当,诊治见效,续用。兼顾肺脾肾三脏,注重阴阳表里的调和,扶助正气的同时,兼顾邪实的存在,使得脾气复升、肾得固摄,故尿血得以改善。